Check & Trace enables fast Salmonella serotyping based on DNA technology
NEW! Check & Trace Salmonella 2.0
Save valuable lab time with our new molecular Salmonella serotyping workflow using qPCR multiplexing technology. Get results in under two hours.
All living organisms are defined by the nature of their genetic code. The last decades has seen a revolution of technologies to determine the complete genetic code of any organism. Likewise, methodologies have been developed that can use these genetic codes to uniquely identify individual organisms and discriminate it from near relatives. We have developed an innovative DNA fingerprinting technology, called “Check & Trace”
to detect and characterise any organism.
Check & Trace is a novel genetic test system to rapidly confirm and identify up to 300 Salmonella serotypes. We offer two solutions to our customers. Our legacy product the Check & Trace Salmonella based on microarray detection and our new Check & Trace Salmonella 2.0 based on real-time PCR. Our legacy product detects up to 300 serotypes and is supported by our tech team in case an undefined genetic code is found. Our new Check & Trace Salmonella 2.0 has a very simple “plug-and-play” workflow giving test results within two hours. It may detect 59 serotypes; the ones mostly encountered in food production area’s.
Proven, industry-leading technology
Check & Trace Salmonella uses proven technology certified by leading industry bodies, including the AOAC Research Institute and the World Organization for Animal Health (OIE).
- AOAC-RI certified for 135 Salmonella serotypes
- International OIE certification for 22 Salmonella serotypes of current and future regulatory significance
Download a complete list of AOAC-RI and OIE certifications.
Note: The number of Salmonella serotypes identified is constantly expanding as we further develop the database linking the spot patterns to specific serotypes.
Trace Salmonella at the source in 3 simple steps
Sampling
Take one single test from pure culture.
Confirm
Discriminate up to 300 Salmonella serotypes through differences in their DNA sequence. Even strains with incomplete profiles can be accurately assigned to a serotype because of the high discriminative power of the software when identifying molecular markers. This is superior to conventional techniques.
Eliminate
Rapid tracing enables the correct actions to be taken to eliminate the source of Salmonella.
Check & Trace Salmonella - see how it works
Research & Whitepapers
Chui et al., (2021)
Microorganisms
“Serotyping using Check & Trace Salmonella is rapid and easy to implement in frontline microbiology as well as public health laboratories and is accurate when compared with traditional methods.”
Diep et al., (2019)
Frontiers in Microbiology
“Compared to the traditional method, the Check & Trace Salmonella assay correctly serotyped 97% of the strains.”
Ferrato et al. (2017)
Journal of Microbiological Methods
“With the shift towards molecular methods for identification, Check & Trace Salmonella provides an easy transition and demonstrates ease-of-use and reduces the turnaround time to result significantly compared to classical serotyping.”
An easy-to-learn
workflow with results
in 8 hours
The workflow uses standard laboratory equipment already available in most microbiology laboratories. Routine implementation is easy following just one day of training. Automated software supports easy interpretation of results.
Sample Prep
- Heating block
- Vortex mixer
30 min
Identification
- Thermocycler
- Mini-centrifuge
- Flow cabinet (opt.)
2 x 15 min
Amplification
- Thermocycler (optional)
15 min
Detection
- Thermomixer with active cooling
- Vortex mixer
- Mini-centrifuge
3 x 15 min
Results
- Heating block
- Vortex mixers
- Check & Trace tube reader with software
- Computer with USB drive and internet connection
- Barcode reader (optional)
30 min
The importance of
Salmonella testing
According to the EU One Health Zoonoses Report, Salmonella is the second most reported foodborne gastrointestinal infection in humans with over 52,000 confirmed cases in 2020.
Salmonella is widespread and difficult to manage
Salmonellosis is not an important disease in poultry. There are two types that cause disease (of the 2600), Gallinarum and Pullorum, but companies can manage these quite well. The problem is other Salmonella types that may cause disease in humans and that often originate from food, mostly fresh meat.
A complete eradication of Salmonella from poultry production is an incredibly difficult goal due to the large variety of serotypes and highly complex epidemiology of this microorganism. In addition:
- The bacteria are widespread in nature and able to survive weeks or months
- Salmonella may constantly be re-introduced in production area’s by contaminated feed, soil, transport systems and materials from other production area’s
- Salmonella may also be attached for longer periods to surfaces and equipment
- Animals will mostly carry Salmonella without any symptoms
The ability to manage Salmonella safely is complex and combines many different elements including biosecurity, diagnostics and vaccination protocols.
Salmonella identified, what next?
Once Salmonella is confirmed, there is an urgent need to identify the serotype. The chain (breeders, hatchery, grow-out and feed) must be interrogated to obtain and serotype the isolates to find the source of the contamination. The faster serotypes are identified, the sooner a treatment strategy can be shaped.
