Verificação & O rastreamento permite a rápida serotipagem de Salmonella com base na tecnologia do DNA
Check & Trace is a novel genetic test system to rapidly confirm and identify 300+ Salmonella serotypes. The test is based on a microarray platform. Each position on the microarray represents a specific DNA marker associated with a unique Salmonella target sequence. Spots only become visible if the DNA markers exactly match the corresponding DNA sequences of the Salmonella isolate. The Check&Trace Software automatically assigns the correct serotype via interpretation of the combination of present and absent spots.
Proven, industry-leading technology
Check & Trace Salmonella uses proven technology certified by leading industry bodies, including the AOAC Research Institute and the World Organization for Animal Health (OIE).
- AOAC-RI certified for 135 Salmonella serotypes
- International OIE certification for 22 Salmonella serotypes of current and future regulatory significance
- ISO-17025 certified (Oli @ Pieter – do we need to go into details here? What areas are DIN certified?)
Download a complete list of AOAC-RI and OIE certifications.
Note: The number of Salmonella serotypes identified is constantly expanding as we further develop the technology.
Trace Salmonella at the
source in 3 simple steps
Sampling
Take one single test from pure culture.
Confirm
Discriminate 300+ Salmonella serotypes through differences in their DNA sequence. Even strains with incomplete profiles can be accurately assigned to a serotype because of the high discriminative power of the software when identifying molecular markers. This is superior to conventional techniques.
Eliminate
Rapid tracing enables the correct actions to be taken to eliminate the source of Salmonella.
Check & Trace Salmonella
Research & Whitepapers
Chui et al., (2021)
Microorganisms
“Serotyping using Check&Trace Salmonella is rapid and easy to implement in frontline microbiology as well as public health laboratories and is accurate when compared with traditional methods.”
Diep et al., (2019)
Frontiers in Microbiology
“Compared to the traditional method, the Check&Trace Salmonella assay correctly serotyped 97% of the strains.”
Ferrato et al. (2017)
Journal of Microbiological Methods
“With the shift towards molecular methods for identification, Check&Trace Salmonella provides an easy transition and demonstrates ease-of-use and reduces the turnaround time to result significantly compared to classical serotyping.”
An easy-to-learn
workflow with results
in 8 hours
The workflow uses standard laboratory equipment already available in most microbiology laboratories. Routine implementation is easy following just one day of training. Automated software supports easy interpretation of results.
Sample Prep
- Heating Block
- Vortex Mixers
30 min
Identification
- Thermocycler
- Mini-centrifuge
- Flow cabinet (opt.)
30 min
Amplification
- Thermocycler (opt.)
15 min
Detection
- Thermomixer with active cooling
- Vortex mixer
- Mini-centrifuge
45 min
Results
- Heating Block
- Vortex Mixers
- Check & Trace tube reader with software
- Computer (USB & internet connection)
- Barcode reader (opt.)
45 min
The importance of
Salmonella testing
According to the EU One Health Zoonoses Report, Salmonella is the second most reported foodborne gastrointestinal infection in humans with over 52,000 confirmed cases in 2020. (Reference)
Salmonella is widespread and difficult to manage
Salmonellosis is not the most devastating poultry disease, but Salmonella is one of the most difficult agents to control.
A complete eradication of Salmonella from poultry production is an incredibly difficult goal due to the large variety of serotypes and highly complex epidemiology of this microorganism. In addition:
- The bacteria are widespread in nature and able to survive weeks or months
- A single flock can be infected with more than one serotype
- Birds can be infected and show no signs of disease
- Pigs can become infected and act as reservoirs of Salmonella.
The ability to manage Salmonella safely is complex and combines many different elements including biosecurity, diagnostics and vaccination protocols.
Salmonella identified, what next?
Once Salmonella is confirmed, there is an urgent need to identify the serotype. The chain (breeders, hatchery, grow-out and feed) must be interrogated to obtain and serotype the isolates to find the source of the infection//contamination. The faster serotypes are identified, the sooner a treatment strategy can be shaped, and the risk of production loss and reduced animal welfare mitigated.
